Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
19701 RNase inhibitor 2000 U -20°C $77
19702 RNase inhibitor 10000 U -20°C $310
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Ribonuclease inhibitor effectively inhibits ribonuclease A type(ribonuclease A, B and C) enzyme activity by binding non-covalently to RNase molecules at a 1:1 ratio. It does not inhibit RNA polymerase, DNA polymerase, RNase H, or RNase T1, and is therefore ideal for use in RT-PCR reactions, in vitro tranlscription, or DNA library construction. Mbiotech RNase Inhibitor is a highly purified, nuclease-free preparation. Each lot is rigorously tested and is guaranteed free of non-specific enodnuclease, exonuclease, and RNase activity.


Human Placental Source


● Protection of mRNA in cDNA synthesis.
● Improvement of protein translation performance.
● Increased yields and activity of polysomes.
● Improvement of in vitro virus replication.
● Improvement of RNA translation in homologous systems.

Unit definition

One unit is defined as the amount of ribonuclease inhibitor required to inhibit by 50% the activity of 5ng of ribonuclease A.

Standard unit assay conditions

Activity is measured by the inhibition of hydrolysis of cytidine 2',3'-cyclic monophosphate by ribonuclease A, where 200 units of ribonuclease inhibitor produces 50% inhibition of 1μg of ribonuclease A.


40 units/μl.

Strorage condition

Avoid multiple freeze/thaw cycles and frequent temperature changes. These fluctuations can greatly affect the product stability.

-Long term(Infrequent use:1-2 times/month):-70°C.

-Short term(Daily/Weekly use):-20°C
If stored for long periods at -20°C or opened frequently during storage, addition of DTT to 5mM final concentration into the reaction mixture is recommended to maintain optimal activity.
Note: Ribonuclease inhibitor is active over a broad pH range but requires a minimum of 1mM DTT to maintain activity.