MBP Excellose® Spin Kit
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
23020 MBP Excellose® Spin Kit 20 preps 2-8°C $175
23050 MBP Excellose® Spin Kit 50 preps 2-8°C $392
  
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INTRODUCTION
MBP(maltose binding protein) Excellose® spin kit provides fast and one step method for small-scale purification of MBP fusion protein. It uses ready-to-use MBP Excellose® spin column and centrifugation for rapid processing of multiple samples.
 
HOW TO USE

MBP(maltose binding protein) Excellose¢ç contains MBP Excellose® spin column, collectionTube, and stock solution.

Protocols
1. Vortex the MBP Excellose® tube and pipet 0.5ml of the slurry to Spin Column, add 0.5 ml of Equilibration Buffer in the Spin Column,
and centrifuge at 700 x g (approx. 2,000 rpm) for 2 min.
2. Discard the eluate, load up to 0.5 ml of cell lysate, centrifuge for 2 min, and collect the flow-through fraction.
3. Add 0.5 ml of Washing Buffer, centrifuge for 2 min, and collect the wash fraction.
4. Repeat step 3, if necessary.
5. Add 0.2 ml of Elution Buffer and centrifuge.
6. Collect the eluate.
 
ODERING INFORMATION

Catalog No
Package
23020
20 columns/pkg
23050
50 columns/pkg
 
FIGURE



MBP fusion protein purification
E. coli lysate containing MBP fusion
protein was loaded to MBP Excellose® Spin column.
1 : E. coli lysate
2 : flow-through fraction
3 : wash fraction
4 : purified MBP