Chelating Excellose® Kit
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
22020 Chelating Excellose® Kit 20 preps 2-8°C $175
22050 Chelating Excellose® Kit 50 preps 2-8°C $392
  
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INTRODUCTION
Chelating Excellose® kit provides fast and one step method for small-scale purification of 6xHis tagged protein. It uses IDA Excellose® spin column containing resin with immobilized Ni2+ ion and centrifugation for rapid processing of multiple samples.
 
HOW TO USE

Chelating Excellose® kit contains Ni2+ charged IDA Excellose®, column, collectionTube, and stock solution.
Protocols
1. Vortex the IDA Excellose tube and pipet 0.5ml of the slurry to Column, add 500 ul of equilibration buffer, and centrifuge at 700 x g (approx. 2,000 rpm) for 2 min.
2. Discard the eluate, load up to 0.5 ml of cell lysate, centrifuge for 2 min, and collect the flow-through fraction.
3. Add 0.5 ml of Washing Buffer, centrifuge for 2 min, and collect the wash fraction.
4. Repeat step 3, if necessary.
5. Add 0.2 ml of Elution Buffer and centrifuge.
6. Collect the eluate.



ORDERING INFORMATION

 Catalog.No 
Package
22020                         
20 columns/pkg
22050                           
50 columns/pkg
FIGURE

6xHis tagged protein purification
E. coli lysate containing 6xHis tagged
protein was loaded to IDA Excellose® spin column.
M. Molecular weight marker
1. E. coli cell lysate
2. Flow-through
3. Eluate