SuperReverse™ All-in-one RT-PCR Premix
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
17500 SuperReverse™ All-in-one RT-PCR Premix 20 ul X 96 rxns -20℃ $273
17501 SuperReverse™ All-in-one RT-PCR Premix 50 ul X 96 rxns -20℃ $477
  
Detail View

Description


MBiotech’s One-step 2X RT-PCR Master Premix is a mixture of ExAct Taq DNA polymerase, M-MLV reverse transcriptase, dNTPs, reaction buffer, and stabilizer. Users can perform RT-PCR by simply adding total RNA or poly(A) RNA as substrate and specific primer pair to the premix. One-step 2X RT-PCR Master Premix also contains glycerol and bromophenol blue as a tracking dye, enabling a direct analysis of PCR products on an analytic gel electrophoresis. Stabilizer in premix enables prolonging a shelf life at -20℃. You do not need to dissolve the mixture prior to RT-PCR reaction as does competitor’ freeze-dried one. The composition is optimized to reduce non-specific amplification and to minimize primer-dimer formation.

Applications


One-step RT-PCR.

QC tests

Performance tests.
 

Storage Condition


Store at -20℃ for one year.
 

Figure1.

 
One-step RT-PCR from Cultured Human Cells
 
1. GAPDH
2. PAI-1
3. erbB2
4. b4 integrin
5. p21Waf1
 
PCR Preparation (20 ul total volume)
100 ng total RNA isolated from human dermal fibroblast
10 ul 2X RT-PCR premix
0.5 ul each primer (10 pmol/ul)
 
PCR Cycle Condition
42℃ 20 min
95℃ 5 min
  94℃ 30 sec
  58℃ 30 sec        x 35 cycles
  72℃ 45 sec
72℃ 5 min

Figure 2.

 
One-step RT-PCR of RNA Viral Genome from Infected Animal

PCR Preparation (20 ul total volume)
100 ng total RNA isolated from viral infected pig blood
10 ul 2X RT-PCR premix
0.5 ul each primer (10 pmol/ul)
 
PCR Cycle Condition
42℃ 20 min
95℃ 5 min
  94℃ 30 sec
  58℃ 30 sec        x 35 cycles
  72℃ 45 sec
72℃ 5 min