Super Premix Sapphire 2X Master Mix
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
17100 Super Premix Sapphire, GoPhorIT Taq Version (2x Mastermix) 1 ml -20°C $47
17101 Super Premix Sapphire, GoPhorIT Taq Version (2x Mastermix) 5 ml -20°C $208
17210 Super Premix Sapphire, ExAct PLUS Taq Version (2x Mastermix) 1 ml -20°C $50
17211 Super Premix Sapphire, ExAct PLUS Taq Version (2x Mastermix) 5 ml -20°C $250
  
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Description

MBiotech’s 2X PCR Master Mix is a mixture of thermostable DNA polymerases, dNTPs, reaction buffer, and stabilizer. Users can perform PCR by simply adding template and primer pair to the premix. 2X PCR Master Mix also contains glycerol and bromophenol blue as a tracking dye, enabling a direct analysis of PCR products on a analytic gel electrophoresis. Stabilizer in premix prevents freezing of PCR premix at -20℃ and prolongs a shelf life of products even at 4℃ or at 37℃. The composition is optimized to reduce non-specific amplification and to minimize primer-dimer formation.

Storage Buffer

1 unit/4 ml thermostable DNA polymerases,  50 mM Tris-HCl, pH9.0, 16 mM (NH4)2SO4, 1.75 mM MgCl2, 2% DMSO, 0.1% Tween 20, 0.1 mg/ml BSA, 0.05% bromophenol blue, 12% glycerol, and stabilizer.

Applications


General PCR, colony PCR, RT-PCR, genomic PCR, high fidelity PCR for a cloning.

QC tests

Performance tests.

Storage Condition

Store at -20℃ for one year.
 

Figures 

 
 
10 fg to 1 ng Template dsDNA (8.5 kbp)
1 ml primer mix (5 pmol each)
20 ml total reaction
 
94℃ 30 sec
58℃ 30 sec       x 30 cycles
72℃ 45 sec
 
5 ml PCR product is analyzed on 1% agarose gel