GoPhorIT™ Taq DNA Polymerase
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
11001 GoPhorIT™ Taq DNA Polymerase 250 U -20 °C $30
11002 GoPhorIT™ Taq DNA Polymerase 500 U -20 °C $50
  
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  11001  




Description

Purified from E. coli strain carrying a plasmid with the cloned gene encoding Thermus aquaticus DNA polymerase. GoPhorITTM Taq DNA polymerase catalyzes 5`-3`synthesis of DNA. The enzyme lacks the 3`-5`exonuclease activity, but possesses 5`-3` exonulcease activity. GoPhorITTM Taq DNA polymerase is ideal for all routine PCR applications.

 

Source

Thermus aquaticus
 

Concentration

5 U/µL
 

Storage buffer

20 mM Tris-HCl (pH 7.9), 100 mM KCl, 0.1 mM EDTA, 0.5 mM PMSF, 1 mM DTT, 50 % Glycerol
 

10X reation buffer with 20mM MgCI2

100 mM Tris-HCl (pH8.3), 500 mM KCl, 20 mM MgCl2, 1% Tween20

PCR Products

As most PCR products amplified with GoPhorITTM Taq have one A added at 3`-termini, if PCR fragments are to be cloned into T/A vectors, the last extension step can be extended to up to 30 min.
 

Storage condition

-20°C
 

Quality control test

PCR, Activity, SDS-PAGE/purity, endonuclease/nickase, Specific performance test
 

Activity unit definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmol of dNTPs into an acid insoluble form in 30 min at 72°C.
 

Purity definition

Single 94 kDa band was visible in SDS-PAGE.
 

Contamination test

Nicking activity, endonuclease and exonuclease activity were not detected after the incubation of 0.5 µg λ-of supercoiled pUC18 DNA, 0.5 µg λ-DNA or 0.5 µg λ-HindIII digest with 25 units of this enzyme for 10 hours at 72°C.
 

Applications

PCR cloning, RT-PCR, Multiplex PCR, SSCP, RFLP, RAPD, AFLP etc

Figures


Fig.1.
PCR-SSCP experiment using GoPhorITTM Taq DNA polymerase with supplied 10 X buffer I . The target locus is VNTR D17S5 on human genomic DNA

Fig.2.
PCR amplification of 3 kb Lambda DNA fragment using GoPhorITTM Taq and Supplied B with serially diluted template DNA from 0.1 ng to 10 fg. M: size marker