2X PCR Premixes
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
17001 2X PCR Premix Sapphire, GoPhorIT Taq version 20 ul X 96 rxns -20℃ $88
17002 2X PCR Premix Sapphire, GoPhorIT Taq version 20 ul X 480 rxns -20℃ $367
17003 2X PCR Premix Sapphire, GoPhorIT Taq version 50 ul X 96 rxns -20℃ $165
17004 2X PCR Premix Sapphire, GoPhorIT Taq version 50 ul X 480 rxns -20℃ $740
  
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Description

MBiotech’s 2X PCR Premix is a mixture of thermostable DNA polymerases, dNTPs, reaction buffer, and stabilizer. Users can perform PCR by simply adding template and primer pair to the premix. 2X PCR Premix also contains glycerol and bromophenol blue as a tracking dye, enabling a direct analysis of PCR products on an analytic gel electrophoresis. Stabilizer in premix prevents freezing of PCR premix at -20℃ and prolongs a shelf life of products even at 4℃ or at 37℃. You do not need to dissolve the mixture prior to PCR reaction as does competitor’ freeze-dried one. The composition is optimized to reduce non-specific amplification and to minimize primer-dimer formation.

1. Convenient experiment 
PCR Premix includes all ingredients for PCR reaction and gel analysis. Users can get the best results by a simple
addition of template and primer pair.
2. Stable formula
Even at 37℃, no prominent decrease of enzyme activity is observed at least for 4 weeks. It is highly stable at  -20℃.
3. Economical price
Ready-to-use premix decreases pricing for PCR reaction when comparing with purchasing ingredients separately.
Plastic rack included in the product can be used as a sample preserve box for PCR products.
4. Reproducible results  
Aliquot mix of PCR premix in 96 PCR tubes or strips from one batch produced and strictly quality controlled
guarantees reproducible results. 

Storage Buffer

1 unit/4 ml thermostable DNA polymerases,  50 mM Tris  HCl( pH9.0), 16 mM (NH4)2SO4, 1.75 mM MgCl2, 2% DMSO, 0.1% Tween 20, 0.1 mg/ml BSA, 0.05% bromophenol blue, 12% glycerol, and stabilizer.

Applications

General PCR, colony PCR, RT-PCR, genomic PCR, high fidelity PCR for a cloning.

QC tests

Performance tests.

Storage Condition

Store at -20℃ for one year , or at 4°C for two month.