qPCR Probe 2X Mastermix Kit
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
18100 qPCR Probe 2X Mastermix Kit 2 X 1.25 ml -20℃ $125
18101 qPCR Probe 2X Mastermix Kit 4 X 1.25 ml -20℃ $250
18102 qPCR Probe 2X Mastermix Kit 10 X 1.25 ml -20℃ $640
18103 qPCR Probe 2X Mastermix Kit 20 X 1.25 ml -20℃ $1,100
  
Detail View
  18100

Description

qPCR probe 2X mastermix Kit is a high-performance reagent designed for superior sensitivity and specificity on all real-time instruments. The kit has been formulated for use with probe-detection technology, including Primetime Assays,  TaqMan®, Scorpion®, Assay On Demand®, allelic discrimination and molecular beacon probes. qPCR probe 2X mastermix Kit employs a hot-start DNA polymerase, for high PCR specificity and sensitivity, Since qPCR probe 2X mastermix Kit possesses no polymerase activity during reaction set-up, the kit greatly reduces non-specific amplification including primer-dimer formation. After pre-heationg, qPCR probe 2X mastermix Kit becomes fully activated and in conjunction with a specially optimized buffer chemistry, generates reliable and highly reproducible data on all real-time PCR instruments.
 
For ease-of-use and added convenience, qPCR probe 2X mastermix Kit is provided as a 2x mastermix containing all the components necessary for real-time PCR, including dNTPs and stabilizers. In addition a separate tube of ROX is provided for optional use.
    
Cat. No. amount
18100 100 x 50ul reaction: 2 x 1.25ml 
18101 200 x 50ul reaction: 4 x 1.25ml
18102 500 x 50ul reaction: 10 x 1.25ml
18103 1000 x 50ul reaction: 20 x 1.25ml  

Storage and Stability

The qPCR probe 2X mastermix Kit is shipped on Dry/Blue Ice. All kit components should be stored at -20℃ upon receipt. Excessive freeze/thawing is not recommended. When stored under optimum conditions, the reagents are stable for a minimum of 6 months from date of purchase.

Quality Control

The qPCR probe 2X mastermix Kit and it’s components are extensively tested for activity, processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination and absence of nucleic acid contamination.

Safety Precautions

Harmful if swallowed, Irritating to eyes, respiratory system and skin. Please refer to the material safety data sheet for further information.

Kit Components

qPCR probe 2 X mastermix Kit
2 X 1.25 ml
4 X 1.25 ml
10 X 1.25 ml
20 X 1.25 ml
25 uM ROX dye
100 ul
200 ul
500 ul
2 X 500 ul

Kit compatibility

The qPCR probe 2X mastermix Kit has been optimized for use with all probe chemistries, including PrimeTime Assays,  TaqMan®, Scorpion®, Assay On Demand®, allelic discrimination and molecular beacon probes.
The qPCR probe 2X mastermix Kit can be used on all real-time PCR instruments.