Plasmid Midi prep/ Maxi prep Kit
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
11525 plasmid Midiprep Kit 25 preps RT $260
11550 plasmid Midiprep Kit 50 preps RT $480
11610 plasmid Maxiprep Kit 10 preps RT $210
11620 plasmid Maxiprep Kit 20 preps RT $400
  
Detail View
  11610  11525

Overview
Plasmid Midi prep Kit

The Plasmid MIDIprep kit has been optimized for the rapid purification of DNA up to 60 ml of bacteria for high-copy number plasmid(up to 120ml of bacteria for low-copy number plasmid).
This kit provides the alkaline lysis reagents and the columns packed with anion-exchanger resin. After the cells lysis, the plasmid DNA is bound to the resin insided the column by a gravity-flow procedure, and the contaminants can be remove with wash buffer. After using this convenient kit, the purified plasmid DNA is suitable for downstream application such as transfection, in vitro transcription and translation, and all enzymatic modification. Column binding capacity is 200~300 ug/ column.
 

Kit Contents

Plasmid DNA Midi Kit
Product
25 preps
50 preps
Storage
Cat. No.
11525
11550
Condition
Cell Resuspension Solution
63 ml
63 ml x 2
RT
Cell Lysis Solution
63 ml
123 ml
RT
Neutralization Solution
90 ml
120 ml + 53 ml
RT
Wash Buffer
25 ml x 2
46 ml x 2
RT
Elution Buffer
27 ml
53 ml
RT
RNase A (50mg/ml)
200 ul
200 ul x 2
4 ℃
MIDI Column
25 ea
50 ea
RT
Protocol
1 ea
1 ea
-
 

Features 

Plasmid DNA Midi Kit
Sample Size : up to 60ml of bacteria for high-copy number plasmid 
                     up to 120ml of bacteria for low-copy number plasmid
Binding Capacity: 200~300 ug/ column


Applications

Isolation of high copy plasmid DNA
Isolation of low copy plasmid DNA

Storage

Kit contents should be stored dry at room temperature. Under these conditions, the kit stable for 12 months.
 

Shipping Conditions

Room temperature.


 

Overview
Plasmid Maxiprep Kit

The Plasmid MAXI prep kit has been optimized for the rapid purification of DNA up to 60-240 ml of bacteria for high-copy number plasmid(up to 200-480 ml of bacteria for low-copy number plasmid).
This kit provides the alkaline lysis reagents and the columns packed with anion-exchanger resin. After the cells lysis, the plasmid DNA is bound to the resin insided the column by a gravity-flow procedure, and the contaminants can be remove with wash buffer. After using this convenient kit, the purified plasmid DNA is suitable for downstream application such as trasfection, in vitro transcription and translation, and all enzymatic modification.
Column binding capacity is 500~600 ug/ column.
 

Kit Contents

Plasmid DNA MAXI Kit
Product
10 preps
20 preps
Storage
Cat. No.
11610
11620
Condition
Cell Resuspension Solution
43 ml x 2
55 ml x 3
RT
Cell Lysis Solution
84 ml
165 ml
RT
Neutralization Solution
115 ml
230 ml
RT
Wash Buffer
12 ml + 25 ml
25 ml x 3
RT
Elution Buffer
15 ml
25 ml
RT
RNase A (50mg/ml)
200 ul x 2
200 ul x 3
4 ℃
MAXI Column
10 ea
20 ea
RT
Protocol
1 ea
1 ea
-
 

Quality Control

The performance of Plasmid MAXI prep Kit is monitored routinely on a lot number. This kit is tested with high-copy plasmid DNA from 40 ml overnight culture in LB medium and yield of plasmid DNA is measured with spectrophotometer. The quality of isolated DNA is checked by restriction digestion, agarose gel electrophoresis, and spectrophotometry.
 

Features

Plasmid DNA Maxi Kit
Sample Size : up to 60 -240ml of bacteria for high-copy number plasmid 
                   up to 200 -480ml of bacteria for low-copy number plasmid
Binding Capacity: 500~600 ug/ column

Applications

Isolation of high copy plasmid DNA
Isolation of low copy plasmid DNA

Storage

Kit contents should be stored dry at room temperature. Under these conditions, the kit stable for 12 months.

Shipping Conditions

Room temperature.