qPCR SYBR Green 2X Mastermix Kit
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
18300 qPCR SYBR Green 2X Mastermix Kit 2 X 1.25 ml -20℃ $125
18301 qPCR SYBR Green 2X Mastermix Kit 4 X 1.25 ml -20℃ $250
18302 qPCR SYBR Green 2X Mastermix Kit 10 X 1.25 ml -20℃ $640
18303 qPCR SYBR Green 2X Mastermix Kit 20 X 1.25 ml -20℃ $1,100
  
Detail View
  18300

Description

The qPCR SYBR Green 2X mastermix Kit is a high-performance reagent designed for superior sensitivity and specificity on various real-time PCR instruments. The qPCR SYBR Green 2X mastermix Kit employs a hot-start DNA polymerase, for high PCR specificity and sensitivity. qPCR SYBR Green 2X mastermix Kit is inactivate and possesses no polymerase activity during the reaction set-up, preventing non-specific amplification including primer-dimer formation.
For ease-of-use and added convenience, qPCR SYBR Green 2X mastermix Kit is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR Green I dye, dNTPs, stabilizers and ROX for optional use. As a ready-to-use premix, only primers and template need to be added.
  
Cat. No. amount
18300 100 x 50ul reaction: 2 x 1.25ml
18301 200 x 50ul reaction: 4 x 1.25ml
18302 500 x 50ul reaction: 10 x 1.25ml
18303   
1000 x 50ul reaction: 20 x 1.25ml


Storage and Stability

The qPCR SYBR Green 2X mastermix Kit is shipped on Dry/Blue Ice. All kit components should be stored at -20℃ upon receipt. Excessive freeze/thawing is not recommended. Since SYBR Green I is light sensitive, it is important to avoid prolonged exposure to light. When stored under optimum conditions, the reagents are stable for a minimum of 6 months from date of purchase.
 

Quality Control

The qPCR SYBR Green 2X mastermix Kit and its components are extensively tested for activity processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination and absence of nucleic acid contamination prior to release.
 

Safety Precautions

Harmful if swallowed, Irritating to eyes, respiratory system and skin. Please refer to the material safety data sheet for further information.
 

Kit Components

qPCR SYBR Green 2X mastermix Kit
2 X1.25ml
4 X1.25ml
10 X 1.25ml
20 X 1.25ml

 
Kit compatibility

 
The qPCR SYBR Green 2X mastermix Kit is optimized for use on the real-time instruments listed in the following compatibility table.
However the kit is also compatible with several instruments that do not require the use of ROX, such as the Qiagen (Corbett) Rotor-GeneTM 6000, the Bio-Rad CFX96 or the Roche LightCycler 480.
 
Manufacturer
Model
ABI
7000, 7300, 7700, 7900, 7900HT and StepOneTM