Hiphi DNA Polymerase
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
14001 Hiphi DNA Polymerase 250 U -20 C $64
14002 Hiphi DNA Polymerase 500 U -20 C $120
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Mbiotech HiPhi DNA Polymerase is an extremely thermostable proofreading DNA polymerase, suitable for applications requiring high temperature synthesis of DNA. DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5 to 3 direction in the presence of Mg2+. It exhibits the 3 to 5 proofreading activity, resulting in over 10-fold higher fidelity than possible with Taq DNA Polymerase


• Ultra pure recombinant protein.
• Recommended for use in high-fidelity amplification, amplification of GC-rich senquences or problematic secondary structures, primer extension reactions at elevated temperatures and cloning of blunt-ended amplification products. 


• Routine PCR applications
• Primer extensions   

10X PCR Buffer without MgCl2

500mM KCl, 100mM Tris-HCl (pH 9.1 at 20C) and 0.1% TritonX-100. The buffer is optimized for use with 0.1 - 0.2mM of each dNTP.

Storage Buffer

20mM Tris-HCl (pH 8.0 at 22C), 100mM KCl, 0.5% Tween 20, 0.5% Nonidet-P40, 0.1mM EDTA, 1mM DTT and 50% glycerol. 

Storage and Stability

The Hiphi DNA Polymerase is shipped on Dry/Blue Ice. All kit components should be stored at -20 upon receipt. Excessive freeze/thawing is not recommended. When stored under optimum conditions, the reagents are stable for a minimum of 6 months from date of purchase.

Quality Control

All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification. 

Safety Precautions

Harmful if swallowed. Irritating to eyes, respiratory system and skin. Please refer to the material safety data sheet for further information. 

Unit Definition

1u is defined as amount of enzyme that required to catalyze the incorporation of 10nmoles of dNTP into acid-insoluble material in 30 minutes at 74C.



Amplification using Hiphi DNA Polymerase
M1: 1 kb DNA Ladder
0.5 and 1.5: 0.5 kb ampification product generated using 0.2 mM dNTPs and 2.0u Pfu DNA Polymerase.
5 and 8: 5 kb and 8 kb amplification products generated using 0.25 mM dNTPs, 2.5u Pfu DNA Polymerase and 3% of formamide.
M2: Lambda / Hind III Marker
0.7% TAE agarose gel