Total RNA Miniprep Kit(Animal tissue sample)
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
15300 Total RNA Miniprep Kit(Animal tissue sample) 50 preps RT $150
15400 Total RNA Miniprep Kit(Animal tissue sample) 200 preps RT $465
  
Detail View
  15300

Overview

The RNA Mini Purification Kit (for tissue sample) is the ideal tool for reliable and fast manual isolation and purification of high quality total RNA from animal tissue and cultured cells. For reproducible high yields and appropriate sample storage and quick operation under the rules for RNA operation is essential. The purified RNA is ready to use for molecular biological analysis. The isolation protocol and all buffers are optimized to assure a high yield as well as a high purity of purified total RNA. All manual work is reduced to a minimum.
The product is indented for use by professional users such as technicians, physicians and biologist trained in molecular biological techniques. It is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification.
 
This method first lyses cells by using a chaotropic salt, then binds RNA to silica-based membranes, washed RNA with ethanol-contained wash buffer and then elutes purified RNA by RNase-free ddH2O. It takes 30 min for an entire procedure, and the purified RNA is ready for RT-PCR, northern blotting, primer extension and cDNA library construction.
 

Specification

Sampling: up to 30 mg tissue
            Up to 1 X 107 animal cultured cells, 1 X 109 bacterial cells, or 5 X 107 yeast
Yield: Up to 40 μg total RNA
Handling time: about 30 minutes
 

Kit Contents

RNA Mini Purification Kit
Product
50 preps
200 preps
Storage
Cat. No.
15300
15400
Condition
TRB Buffer
25 ml
100 ml
RT
Wash Buffer I
30 ml
120 ml
RT
Wash Buffer II (conc.)
15 ml
35 ml x 2
RT
RNase-free ddH2O
6 ml
12 ml
RT
Filter Column
50 ea
200 ea
RT
Column
50 ea
200 ea
RT
2.0ml Collection Tube
100 ea
400 ea
RT
Elution Tube
50 ea
200 ea
RT
Micropestle
50 ea
200 ea
RT
Quick Guide
1 ea
1 ea
-
 

Quality Control

RNA Mini Purification Kit can be guaranteed the correct function for applications as described in the manual. In accordance with Mbiotech’s certified Quality Management System each component of the RNA Mini Purification Kit were tested against predetermined specifications to ensure consistent product quality.
 

Storage Condition

All buffers and kit contents should be stored at room temperature (RT) and are stable for at least 12 months under these conditions.
Room temperature is defined as range from 15-25°C.
 
Sample
Recommended amount of sample used
RNA yield(ug)
Animal cells
(up to 5 x 106)
NIH/3T3
HeLa
COS-7
LMH
1x106 cells
1x106 cells
1x106 cells
1x106 cells
10
15
30
12
Animal Tissues
(Mouse/rat)
(up to 30 mg)
Embryo
Heart
Brain
Kidney
Liver
Spleen
Lung
Thymus
10 mg
10 mg
10 mg
10 mg
10 mg
10 mg
10 mg
10 mg
25
10
10
30
50
35
15
45
Bacteria
E.coli
B.subtilis
1x109cells
1x109 cells
60
40
Yeast
(up to 5x107)
S.cerevisiae
1x107 cells
25