Genomic DNA purification Kit(Bacterial/Yeast sample)
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
16050 Genomic DNA purification Kit(Bacterial/Yeast sample) 50 preps RT $125
16200 Genomic DNA purification Kit(Bacterial/Yeast sample) 200 preps RT $400
  
Detail View
  16050

Overview

The Genomic DNA purification kit (for bacteria/yeast sample) is designed for rapid isolation of high quality genomic DNA from both Gram positive,  Gram negative bacteria and yeast.  It provides a fast, simple and inexpensive method requiring no expensive equipment, hazardous reagents and involving a few steps. The Genomic DNA purification kit use advanced silica-based membrane technology for rapid and efficient purification of genomic DNA without organic extraction or ethanol precipitation. DNA purified with this system is suitable for a variety of application, including amplifications, membrane hybridizations, and digestion with restriction endonuclease.
For reproducible and high yields appropriate sample storage is essential. The purified DNA can be used for molecular biological analysis.
The product is intended for use by professional users such as technicians, physicians and biologists trained in molecular biological techniques. It is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modification of DNA followed by signal detection or amplification. Any molecular biological results generated using the sample preparation procedure in conjunction with any downstream molecular biological assay should be interpreted with regard to other clinical or laboratory findings. To minimize irregularities in molecular biological results, adequate controls for downstream applications should be used.
This technology first lyses cells and degrades protein by using a chaotropic salt and Proteinase K, then binds DNA to silica based membranes, washes DNA with ethanol contained wash buffer and then elutes purified DNA by low salt elution buffer or ddH2O. Compare with other harmful and time-consuming procedures, this kit shortens the handling time within 1 hour (lysis time varies depending on the type of tissue processed). The size of purified DNA is up to 50 kb (predominantly 20 – 30 kb). DNA of this length performs the highest efficiency during PCR reaction. After using this kit, the high quality total DNA can be used directly for the down-stream applications.
 
  
 

Kit Contents

Genomic DNA Purification Kit (for bacteria/yeast)
Product
50 preps
200 preps
Storage
Cat. No.
16050
16200
Condition
BYG I Buffer
10 ml
40 ml
RT
BYG II Buffer
11 ml
44 ml
RT
Wash Buffer 1 (conc.)
16 ml 33 ml X 2 RT
Wash Buffer 2 (conc.)
11 ml 22 ml X 2 RT
Elution Buffer
15 ml
23 ml
RT
Proteinase K
1 ml
1 ml X 4
4 ℃
Column
50 ea
200 ea
RT
2.0ml Collection Tube
50 ea
200 ea
RT
Protocol
1 ea
1 ea
 
 
 

Quality Control

The performance of Genomic DNA Purification Kit is monitored routinely on a lot number. The quality of isolated DNA is checked by restriction digestion, agarose gel electrophoresis, and spectrophotometry.
 

Features

  • Sample Amount : up to 25mg of fresh or frozen tissue
  • Yield : up to 50ug of total DNA, depends on the sample types
  • Handling time : 30 -60 min, depends on the sample types
 

Applications

Isolation of total DNA from
  • Tissue
  • Fixed Tissue
  • Bacteria
  • Yeast
  • Dried Blood Spot
 

Storage

Kit contents should be stored dry at room temperature. Under these conditions, the kit is stable for 12 months.
 

Storage Condition

All buffers and kit contents should be stored at room temperature (RT) and are stable for at least 12 months under these conditions.
The Proteinase K and RNase A can be stored at room temperature or at 2–8°C but recommended storage temperature is -20℃.
Room temperature is defined as range from 15-25°C.