Plasmid Miniprep kit
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
11200 Plasmid Miniprep kit 200 prep RT $135
  
Detail View
  11200


Overview

The Plasmid Miniprep Kit provides a fast and simple method for purifying plasmid DNA from bacterial cultures. Plasmid Miniprep Kit contains chaotropic salt that enhances the binding of DNA onto a glass surface and reagents for alkaline lysis to purify easily plasmid DNA from bacteria. The column is packed with glass fiber and has enough surface area to bind up to 20-30µg of high copy plasmid DNA. Phenol extraction and ethanol precipitation are not required. The Plasmid Miniprep kit shortens the handling time to about 25 minutes. Purified plasmid DNA is ready for restriction enzyme digestion, ligation, transformation, sequencing reactions, and Library screening.

Kit Contents

Plasmid Miniprep Kit
Product
200 preps
Storage
Cat. No.
11200
Condition
Cell resuspension Solution
60 ml
RT
Cell Lysis Solution
60 ml
RT
Neutralization Solution
80 ml
RT
Wash Buffer (conc.)
20 ml
RT
Elution Buffer
30 ml
RT
RNase A (25mg/ml)
200 ul
4 ℃
Column
200 ea
RT
Collection Tube
200 ea
RT
Protocol
1
-
 

Features

1. Sampling : 1 - 5ml overnight culture.
2. Yield : 20ug - 30ug of high-copy plasmid
3. Plasmid Size : < 12kb
4. Handling Time : about 25min

Applications

Isolation of plasmid DNA

Storage

Kit contents should be stored dry at room temperautre. Under these conditions, the kit is stable for 12 months.
 

Shipping Conditions

Room temperature.